The two teams have only faced each other once in the EFL Cup, with Colchester United defeating Brentford 1-0 in the second round of the 2019/20 competition.
Colchester United's home ground is the JobServe Community Stadium, while Brentford's home ground is the Brentford Community Stadium.
Colchester United's most successful EFL Cup campaign came in the 1974/75 season when they reached the quarter-finals, while Brentford's best performance was reaching the fourth round in the 1961/62 season.
Both teams have a long history in English football, with Colchester United having spent most of their history in the lower divisions, while Brentford has fluctuated between the lower and upper tiers of the English league system.